Induction of callus and plant regeneration in Vicoa indica

Thulaseedharan, A and Vaidyanathan, CS (1990) Induction of callus and plant regeneration in Vicoa indica. In: Plant Cell, Tissue and Organ Culture, 23 (1). pp. 45-48.

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Abstract

Callus cultures were initiated from the stem and leaf explants of aseptically grown Vicoa indica. A simple method is described for plant regeneration from callus and the rapid multiplication of the plants thus obtained. Callus initiation was optimum in Gamborg B5 (B5) basal medium containing either $2.0 mg\hspace{2mm} l^{-1}$ naphthaleneacetic acid (NAA) with $0.2 mg\hspace{2mm} l^{-1}$ kinetin (Kn) or $2.0 mg\hspace{2mm} l^{-1}$ 6-benzylaminopurine (BAP) with $0.2 mg\hspace{2mm} l^{-1}$ NAA. The calli initiated on B5 medium were able to proliferate on both Murashige and Skoog (MS) and B5 basal medium. Shoot primordia were obtained from greenish callus on passage to B5 basal medium containing $3.0 mg\hspace{2mm} l^{-1}$ BAP and $1.0 mg\hspace{2mm} l^{-1}$ Kn. On further subculture onto B5 medium containing $0.2 mg\hspace{2mm} l^{-1}$ Kn the shoot primordia developed into plantlets.

Item Type:	Journal Article
Publication:	Plant Cell, Tissue and Organ Culture
Publisher:	Kluwer Academic Publishers
Additional Information:	Copyright of this article belongs to Kluwer Academic Publishers.
Keywords:	callus induction;micropropagation;plant regeneration;Vicoa indica
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	21 Dec 2007
Last Modified:	19 Sep 2010 04:41
URI:	http://eprints.iisc.ac.in/id/eprint/12535

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