The hemagglutinin–neuraminidase protein of peste des petits ruminants virus is biologically active when transiently expressed in mammalian cells

Seth, Shaguna and Shaila, MS (2001) The hemagglutinin–neuraminidase protein of peste des petits ruminants virus is biologically active when transiently expressed in mammalian cells. In: Virus Research, 75 (2). pp. 169-177.

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Abstract

The genes coding for the surface glycoproteins hemagglutinin–neuraminidase (HN) of the peste des petits ruminants virus (PPRV) and hemagglutinin (H) of rinderpest virus (RPV) were cloned in a cytomagalovirus promoter driven expression vector and expressed transiently in mammalian cells. The protein expression was apparent 24 h after transfection and the expressed proteins were detected at the cell surface. The transiently expressed PPRV HN protein was found to be biologically active in possessing hemadsorption and neuraminidase activities. On the other hand, RPV H protein exhibited neuraminidase activity but was deficient in hemadsorption activity. The substrate specificity of the neuraminidase activity of these two proteins differed distinctly. The presence of neuraminidase activity in both PPRV HN and RPV H proteins is unusual among members of the morbillivirus genus.

Item Type:	Journal Article
Publication:	Virus Research
Publisher:	Elsevier
Additional Information:	Copyright of this article belongs to Elsevier.
Keywords:	Peste des petits ruminants virus;Rinderpest virus;Hemagglutinin–neuraminidase protein;Hemadsorption; Neuraminidase;Fusion promotion
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	15 Oct 2007
Last Modified:	19 Sep 2010 04:40
URI:	http://eprints.iisc.ac.in/id/eprint/12254

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