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Characterization of monoclonal antibodies to glycodelin and recombinant glycodelin

Karri, SriTulasi and Mukhopadhyay, Debaditya and Jing, Liu and Karande, Anjali A (2000) Characterization of monoclonal antibodies to glycodelin and recombinant glycodelin. In: The Histochemical Journal, 32 (12). pp. 711-716.

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Glycodelin-A belongs to the lipocalin superfamily. Although it is associated with normal endometrial growth during the menstrual cycle, fertilization and normal pregnancy in humans, the molecular mechanism of its biological action has not been elucidated. To undertake studies to understand the functional relevance of any molecule, obtaining large quantities of the protein becomes essential. With the ultimate aim of purifying glycodelin either from its natural sources (human amniotic fluid) or the recombinant glycodelin from bacterial recombinant lysates, we raised monoclonal antibodies to this protein. As immunogens, recombinant glycodelin expressed in E. coli and Pichia pastoris as well as glycodelin from amniotic fluid were used. The monoclonal antibodies generated were characterized with respect to binding to both the native as well as the recombinant proteins using ELISA, immunoblotting, and immunohistochemistry.

Item Type: Journal Article
Publication: The Histochemical Journal
Publisher: Kluwer
Additional Information: Copyright of this article belongs to Kluwer.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 17 Oct 2007
Last Modified: 19 Sep 2010 04:40
URI: http://eprints.iisc.ac.in/id/eprint/12224

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