Incorporation of oxyethylene units between hydrocarbon chain and pseudoglyceryl backbone in cationic lipid potentiates gene transfection efficiency in the presence of serum

Dileep, PV and Antony, A and Bhattacharya, Santanu (2001) Incorporation of oxyethylene units between hydrocarbon chain and pseudoglyceryl backbone in cationic lipid potentiates gene transfection efficiency in the presence of serum. In: FEBS-Letters, 509 (2). pp. 327-331.

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Abstract

Four novel cationic lipids with different numbers of oxyethylene units at the linkage region between the pseudoglyceryl backbone and the hydrocarbon chains have been synthesized and used as mixtures with 1,2-dioleoyl- -α-glycero-3-phosphatidyl ethanolamine (DOPE) for liposome-mediated gene transfection. Incorporation of different numbers of oxyethylene (–CH2CH2O–) units between long hydrocarbon chain at the C-1 and C-2 positions of the pseudoglyceryl skeleton improved the transfection efficiency considerably compared to the one in which the chains were connected via simple ether links. A pronounced improvement in the gene transfer efficiency was observed with the unsymmetrical cationic lipid 3 in which the long hydrocarbon at the C-1 position of the pseudoglyceryl segment is connected via two (–CH2CH2O–) units. Notably, the transfection ability of lipid 3 with DOPE in the presence of serum was significantly greater than LIPOFECTAMINE®. This suggests that introduction of oxyethylene units between long hydrocarbon chains at the C-1 and C-2 positions of the pseudoglyceryl skeleton provides a novel strategy to achieve efficient gene transfer, especially in conditions where the presence of serum is critical.

Item Type:	Journal Article
Publication:	FEBS-Letters
Publisher:	Elsevier Science B.V.
Additional Information:	Copyright of this article belongs to Elsevier Science B.V.
Keywords:	Cationic liposome;Cationic lipid;Oxyethylene linkage;DNA transfection
Department/Centre:	Division of Chemical Sciences > Organic Chemistry Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	23 Oct 2007
Last Modified:	19 Sep 2010 04:40
URI:	http://eprints.iisc.ac.in/id/eprint/12198

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