Sequence Analysis of VP4 and VP7 Genes of Nontypeable Strains Identifies a New Pair of Outer Capsid Proteins Representing Novel P and G Genotypes in Bovine Rotaviruses

Rao, Durga C and Gowda, Krishne and Reddy, Yugandhar BS (2000) Sequence Analysis of VP4 and VP7 Genes of Nontypeable Strains Identifies a New Pair of Outer Capsid Proteins Representing Novel P and G Genotypes in Bovine Rotaviruses. In: Virology, 276 (1). pp. 104-113.

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Abstract

During a limited epidemiological study, the serotype specificities of several isolates of bovine rotavirus, exhibiting identical electropherotypes, from a single cattle farm near Bangalore, India, could not be determined using a panel of serotyping monoclonal antibodies (MAbs) specific for G serotypes 1–6 and 10. To determine the genotypes of these isolates, the nucleotide sequences of the genes encoding the outer capsid proteins VP4 and VP7 of two representative isolates, Hg18 and Hg23, were determined. The corresponding gene sequences from the two isolates were identical, indicating that these isolates represented a single strain of bovine rotavirus. Comparison of the VP4 nucleotide (nt) and the deduced amino acid (aa) sequences with those of several human and animal rotavirus strains representing all of the currently recognized 20 different VP4 (P) genotypes revealed low nt and aa sequence identities of 61.0 to 74.2% and 57.9 to 78.2% for VP4. The percentages of amino acid homology for the VP8* and VP5* regions of VP4 were 37.7 to 67.9 and 68.1 to 84.2%, respectively. The nt and aa sequences of the VP7 gene were also distinct from those of human and animal strains belonging to the previously established 14 VP7(G) serotypes (65.9 to 75.5% nt and 59.5 to 77.6% aa identities). These findings suggest the classification of the VP4 and VP7 genes of the bovine isolates represented by Hg18 as new P and G genotypes and provide further evidence for the vast genetic/antigenic diversity of group A rotaviruses.

Item Type:	Journal Article
Publication:	Virology
Publisher:	Academic Press
Additional Information:	Copyright of this article belongs to Academic Press.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	09 Oct 2007
Last Modified:	19 Sep 2010 04:40
URI:	http://eprints.iisc.ac.in/id/eprint/12178

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