Identification of lysophosphatidic acid acyltransferase in the microsomal membranes of developing castor endosperm

Ajay, Tumaney W and Narkunaraja, S and Ram, Rajasekharan (1999) Identification of lysophosphatidic acid acyltransferase in the microsomal membranes of developing castor endosperm. In: Current Science, 76 (5). pp. 660-664.

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Abstract

A photoreactive substrate analog of acyl-CoA, 12-((4-azidosalicyl)amino)dodecanoyl-CoA (ASD-CoA) acts as a competitive inhibitor of acyl-CoA : lysophosphatidic acid acyltransferase in the microsomal membranes of developing castor endosperm (Ricinus communis L.). The acyltransferase was irreversibly inactivated when the microsomal membranes were exposed to ultraviolet light in the presence of ASD-CoA. The substrate, oleoyl-CoA was able to protect the enzyme against photoinactivation. Analysis by SDS-PAGE followed by autoradiography of microsomal membrane photolysed with 125I-labelled ASD-CoA revealed major labelling of a single protein with the molecular mass of 29 kDa, suggesting that this protein could be the putative LPA acyltransferase.

Item Type:	Journal Article
Publication:	Current Science
Publisher:	Indian Academy of Sciences
Additional Information:	copyright of this article belongs to Indian Academy of Sciences
Keywords:	iodine 125 labeling;radionuclide method;SDS PAGE;SDS polyacrylamide gel electrophoresis;analytical method
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	18 Oct 2007
Last Modified:	19 Sep 2010 04:40
URI:	http://eprints.iisc.ac.in/id/eprint/12136

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