Characterization and hormonal modulation of immunoreactive thiamin carrier protein secreted by adult rat leydig cells in vitro

Subramanian, S and Adiga, PR (1999) Characterization and hormonal modulation of immunoreactive thiamin carrier protein secreted by adult rat leydig cells in vitro. In: Journal of Endocrinology, 162 (1). pp. 49-56.

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Abstract

Leydig cells isolated from adult rats and maintained under defined conditions in culture secrete a protein of molecular weight $(M_r)$ 70 000 which is immunologically similar to chicken thiamin carrier protein (TCP). Synthesis of immunoreactive TCP by these cells is demonstrated by immunoprecipitation of $[^{35}S]$methionine incorporated, newly synthesized proteins with monoclonal and polyclonal antibodies to chicken TCP. The amount of immunoreactive TCP secreted into the culture supernatant is quantitated by using a specific radioimmunoassay. Under the influence of LH, secretion of immunoreactive TCP is enhanced 3-fold and can be inhibited by up to 70% with aromatase inhibitor (1,4,6-androstatrien-3,17-dione). Cyclic AMP acts as a second messenger in the sequence of events involved in LH-induced elevation of immunoreactive TCP in Leydig cells. The effects of exogenous estradiol-$17 \beta$ and diethylstilbestrol are comparable in terms of stimulation of secretion of immunoreactive TCP by thesecells. Tamoxifen brought about a 70% decrease in the elevated levels of immunoreactive TCP. These results suggest that estrogen mediates immunoreactive TCP induction in hormonally stimulated adult rat Leydig cells.

Item Type:	Journal Article
Publication:	Journal of Endocrinology
Publisher:	Society for Endocrinology
Additional Information:	Copyright of this article belongs to Society for Endocrinology.
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	17 Oct 2007
Last Modified:	01 Mar 2012 05:40
URI:	http://eprints.iisc.ac.in/id/eprint/12135

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