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C-Phycocyanin: A Potent Peroxyl Radical Scavenger in Vivo and in Vitro

Bhat, Vadiraja B and Madyastha, KM (2000) C-Phycocyanin: A Potent Peroxyl Radical Scavenger in Vivo and in Vitro. In: Biochemical and Biophysical Research Communications, 275 (1). pp. 20-25.

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C-Phycocyanin (from Spirulina platensis) effectively inhibited $CC1_4$-induced lipid peroxidation in rat liver in vivo. Both native and reduced phycocyanin significantly inhibited peroxyl radical-induced lipid peroxidation in rat liver microsomes and the inhibition was concentration dependent with an $IC_{50}$ of 11.35 and $12.7 \mu M$, respectively. The radical scavenging property of phycocyanin was established by studying its reactivity with peroxyl and hydroxyl radicals and also by competition kinetics of crocin bleaching. These studies have demonstrated that phycocyanin is a potent peroxyl radical scavenger with an $IC_{50}$ of $5.0 \hspace{2mm} \mu M$ and the rate constant ratios obtained for phycocyanin and uric acid (a known peroxyl radical scavenger) were 1.54 and 3.5, respectively. These studies clearly suggest that the covalently linked chromophore, phycocyanobilin, is involved in the antioxidant and radical scavenging activity of phycocyanin.

Item Type: Journal Article
Publication: Biochemical and Biophysical Research Communications
Publisher: Academic Press
Additional Information: Copyright of this article belongs to Academic press.
Keywords: Spirulina platensis;C-phycocyanin;Reduced phycocyanin;Peroxyl radical scavenger;Hepatotoxicity
Department/Centre: Division of Chemical Sciences > Organic Chemistry
Date Deposited: 08 Oct 2007
Last Modified: 19 Sep 2010 04:40
URI: http://eprints.iisc.ac.in/id/eprint/12124

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