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A novel TATA-box-binding factor from the silk glands of the mulberry silkworm, Bombyx mori

Srinivasan, Lakshmi and Gopinathan, Karumathil P (2002) A novel TATA-box-binding factor from the silk glands of the mulberry silkworm, Bombyx mori. In: Biochemical Journal, 363 (3). pp. 503-513.

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The presence of one or more TATATAA motifs in the flanking sequences of individual members of a multi-gene $tRNA_1^{Gly}$ family from the mulberry silkworm, Bombyx mori, negatively modulated the transcription of the gene copies. Characterization of proteins from posterior silk gland nuclear extracts, binding to the TATATAA motif, identified a novel 43 kD protein, designated here as P43 TATA-box-binding factor (TBF). The protein was purified to homogeneity. P43 TBF binding was highly sequence-specific and showed a 100-fold-higher affinity for binding than the TATA-box-binding protein (TBP). The protein also showed binding to the TATAAA sequence of the actin5C promoter. P43 TBF inhibited transcription of all the tRNA genes examined, as well as RNA polymerase II transcription from the actin5C promoter. The amino acid sequence of eleven peptides generated from P43 TBF did not share homology with proteins that bind the TATA box, such as TBP, TRF (TBP-related factor) or TLFs (TBP-like factors) reported from other sources. Inhibition of transcription of tRNA genes by P43 TBF could not be reversed by TBP. The inhibitory effect appeared to be exerted through sequestration of the associated transcription factors.

Item Type: Journal Article
Publication: Biochemical Journal
Publisher: The Biochemical Society
Additional Information: Copyright of this article belongs to The Biochemical Society.
Keywords: RNA polymerase III;TATA binding protein;tRNA transcription;Transcriptional regulation
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 05 Oct 2007
Last Modified: 19 Sep 2010 04:40
URI: http://eprints.iisc.ac.in/id/eprint/12098

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