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Embryonic ecdysteroids in a mole crab, Emerita asiatica (Milne-Edwards)

Subramoniam, T and Tirumalai, R and Gunamalai, V and Hoffmann, KH (1999) Embryonic ecdysteroids in a mole crab, Emerita asiatica (Milne-Edwards). In: Journal of Biosciences, 24 (1). pp. 91-96.

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Using radioimmunoassay (RIA) and high performance liquid chromatography (HPLC), the presence of a complex mixture of free and conjugated ecdysteroids is reported in the embryonated eggs of a mole crab, Emerita asiatica. From an initial low value of 6.5 ng/g egg wet weight in stage I, the total ecdysteroids increased in concentration to 15.2 ng/g egg wet weight in stage III. This was followed by a sharp fall in stage IV, but again increased to 15.0 ng/g egg wet weight in stage VI. After a further decline in stage VII, the total ecdysteroids registered the highest value of 36.2 ng/g egg wet weight in stage VIII. This value, however declined to a low level in the prehatching stage (IX). The concentration of the free ecdysteroids always predominated over the conjugated ones. The HPLC analysis of free ecdysteroids demonstrated the presence of 20-hydroxyecdysone and ecdysone in the ratio of 2.5. purified lipovitellin II also contained free and conjugated ecdysteroids. The functional significance of the embryonic ecdysteroids as well as their nature of synthesis and storage within the egg is discussed in the light of the information available on insect embryogenesis

Item Type: Journal Article
Publication: Journal of Biosciences
Publisher: Indian Academy of Sciences
Additional Information: copyright of this article belongs to Indian Academy of Sciences.
Keywords: ecdysteroids;embryogenesis;Emerita asiatica
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited: 17 Oct 2007
Last Modified: 07 Feb 2012 06:07
URI: http://eprints.iisc.ac.in/id/eprint/12083

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