Characterization and hormonal modulation of immunoreactive thiamin carrier protein in immature rat Sertoli cells in culture

Subramanian, Sarada and Adiga, Radhakantha P (1999) Characterization and hormonal modulation of immunoreactive thiamin carrier protein in immature rat Sertoli cells in culture. In: Journal of Steroid Biochemistry and Molecular Biology, 68 (1-2). pp. 23-30.

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Abstract

Immature rat Sertoli cells synthesize and secrete a protein species which has immunological similarity with chicken egg thiamin carrier protein (TCP) as assessed by immunocytochemical localization, liquid phase radioimmunoassay (RIA), immunoprecipitation of $[^{35}S]$-methionine incorporated newly synthesized proteins by polyclonal antibodies (pAbs) to chicken TCP and tryptic peptide mapping of iodinated immunoprecipitated proteins. FSH and testosterone together bring about 4-fold induction of Sertoli cell TCP over the control levels which is inhibitable upto 75% by an aromatase inhibitor. Addition of optimal concentrations of exogenous estradiol-$17 \beta$ to the cultures causes 2-fold enhancement of secretion of TCP which can significantly be inhibited by tamoxifen, when added along with estradiol-$17 \beta$. These results show that Sertoli cells produce estrogen-inducible TCP, presumably to transport the vitamin to the developing germ cells.

Item Type:	Journal Article
Publication:	Journal of Steroid Biochemistry and Molecular Biology
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	04 Oct 2007
Last Modified:	01 Mar 2012 07:00
URI:	http://eprints.iisc.ac.in/id/eprint/12063

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