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Crystallization and preliminary investigations on a telomeric repeat sequence $C_4A_2C_4A_2$

Savitha, G and Leonidas, D and Acharya, KR and Viswamitra, MA (2001) Crystallization and preliminary investigations on a telomeric repeat sequence $C_4A_2C_4A_2$. In: Acta Crystallographica Section D Biological Crystallography, 57 (6). pp. 873-875.

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Repeat units based on the telomeric sequence of the ciliated protozoan Tetrahymena, d$(C_4A_2)2,$ have been crystallized. Cytosine-rich DNA stretches are known to reside in telomeres and centromeres of eukaryotic chromosomes, playing crucial roles in the structural stability of the chromosome in addition to their connection with cancer and aging. Preliminary investigations on the telomeric repeat sequence $C_4A_2C_4A_2$ from CD studies and X-ray crystal data suggest it to be a right-handed interdigitated tetraplex structure with hemiprotonated $C\bullet\hspace{1mm}C^+$ base pairs. The molecules appear to be packed one on top of another forming a discontinuous helix along c simulating a poly-C fibre, an arrangement which maximizes the number of cytosines stacked.

Item Type: Journal Article
Publication: Acta Crystallographica Section D Biological Crystallography
Publisher: International Union of Crystallography
Additional Information: copyright of this article belongs to International Union of Crystallography
Keywords: Biochemistry;Molecular Biophysics;Methods and Techniques
Department/Centre: Division of Physical & Mathematical Sciences > Physics
Date Deposited: 03 Oct 2007
Last Modified: 23 Feb 2012 07:30
URI: http://eprints.iisc.ac.in/id/eprint/12033

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