Gupta, CS and Dighe, RR (2000) Biological activity of Single chain Choriogonadotropin, $hCG \alpha \beta$ is decreased upon deletion of five Carboxyl terminal amino acids of the $\alpha$ subunit without affecting its receptor binding. In: Journal of Molecular Endocrinology, 24 (2). pp. 157-164.
PDF
Biological_activity_of_single_chain_chorionic.pdf Restricted to Registered users only Download (122kB) | Request a copy |
Abstract
The strategy of translationally fusing the two subunits of human Choriogonadotropin (hCG) has been used to produce recombinant single chain hCG in which the C terminus of the a subunit is fused to the N terminus $\beta$ without any linker using Pichia pastoris expression system. The Pichia expressed $hCG\alpha \beta$ $(phCG\alpha \beta)$ attained an overall conformation similar to that of hCG, could bind to the receptor and elicit biological response suggesting that receptor binding and signal transduction can take place even with a molecule having blocked C-terminus of the $\alpha$ subunit. The carboxyl terminal of the $\alpha$ subunit has been shown to be involved in hormone binding and signal transduction of all the heterodimeric glycoprotein hormones. However, deletion of five amino acids from the C-terminus of the $\alpha$ subunit in the single chain hCG did not alter the overall conformation of the fusion molecule and its receptor binding ability, but led to a significant reduction in its ability to elicit biological response. These data show that these five amino acids at the C-terminus of the $\alpha$ subunit in the single chain hCG are not absolutely essential for attaining a conformation required for receptor binding, but are essential for obtaining a full biological response.
Item Type: | Journal Article |
---|---|
Publication: | Journal of Molecular Endocrinology |
Publisher: | Society for Endocrinology |
Additional Information: | Copyright of this article belongs to Society for Endocrinology |
Keywords: | Biochemistry and Molecular Biophysics |
Department/Centre: | Division of Biological Sciences > Molecular Reproduction, Development & Genetics |
Date Deposited: | 01 Oct 2007 |
Last Modified: | 19 Sep 2010 04:39 |
URI: | http://eprints.iisc.ac.in/id/eprint/12018 |
Actions (login required)
View Item |