Agrobacterium-mediated transformation of tomato (Lycopersicon esculentum var. pusa ruby) with coat-protein gene of Physalis mottle tymovirus

Sree, Vidya CS and Manoharan, M and Kumar, Ranjit CT and Savithri, HS and Sita, Lakshmi G (2000) Agrobacterium-mediated transformation of tomato (Lycopersicon esculentum var. pusa ruby) with coat-protein gene of Physalis mottle tymovirus. In: Journal of Plant Physiology, 156 (1). pp. 106-110.

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Abstract

Transgenic tomato plants harboring the coat protein (CP) gene of Physalis mottle tymovirus (PhMV) were obtained through Agrobacterium-mediated genetic transformation. The cotyledonary leaves were transformed by cocultivation with Agrobacterium strain LBA4404 carrying the binary vector pBI 121. The CP gene of PhMV was cloned into the binary vector by deleting the GUS gene. Cotyledonary explants were cultured on the regeneration medium supplemented with 50 mg/L kanamycin and 400 mg/L cefotaxime (MS 1). Shoots regenerated via callus. Putative transformants were transferred to a rooting medium containing 25 mg/L kanamycin (MS2), where successful rooting was obtained. Transgenic nature of the transformants was checked by PCR and Western analyses. The T[1] progenies segregate into a 3 :1 ratio. Southern analysis confirmed the integration of CP gene into the tomato genome of T[1] progenies. The transgenic tomato plants were further challenged with PhMV and exhibited delay in symptom development, thus confirming the establishment of transgenic plants with partial resistance to the virus

Item Type:	Journal Article
Publication:	Journal of Plant Physiology
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	Tomato;coat protein;Physalis mottle tymovirus;Agrobacterium; genetic transformation;disease resistance;Thidiazuron
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	15 Oct 2007
Last Modified:	03 Feb 2012 09:30
URI:	http://eprints.iisc.ac.in/id/eprint/12011

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