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Transformation of peanut (Arachis hypogaea L.): a non-tissue culture based approach for generating transgenic plants

Rohini, VK and Rao, Sankara K (2000) Transformation of peanut (Arachis hypogaea L.): a non-tissue culture based approach for generating transgenic plants. In: Plant Science, 150 (1). pp. 41-49.

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Transgenic peanut (Arachis hypogaea L. cv. TMV-2) plants have been produced by a tissue culture-independent Agrobacterium tumefaciens-mediated transformation procedure. Embryo axes of mature seeds with one cotyledon excised were incubated on Murashige and Skoog (MS) gelled medium for 2 days, then pricked randomly with a sterile needle and infected by immersion in a suspension of Agrobacterium in Winans’ AB medium that was added with wounded tobacco leaf extract. Agrobacterium strain LBA 4404 harboring the binary vector pKIWI105 that carries the genes for $\beta$-glucuronidase (GUS) and neomycin phosphotransferase (NPT II) was used for transformation. Following a 16 h infection, 18 h decontamination with cefotaxime and germination and growth on soilrite for 16 days under growth room conditions, the seedlings were transferred to greenhouse. About 3.3% of the seedlings were GUS positive as determined by histochemical assay and by PCR analysis. Molecular characterization of primary transformants as well as the $T_1$ and $T_2$ generation plants has shown that the method ensured insertion, expression and inheritance of foreign genes in peanut.

Item Type: Journal Article
Publication: Plant Science
Publisher: Elsevier
Additional Information: Copyright of this article belongs to Elsevier.
Keywords: Arachis hypogaea L.;Genetic transformation;Tissue culture-independent method;Agrobacterium tumefaciens;β-Glucuronidase;Neomycin phosphotransferase
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 01 Oct 2007
Last Modified: 19 Sep 2010 04:39
URI: http://eprints.iisc.ac.in/id/eprint/11988

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