A new ratiometric fluorescence probe as strong sensor of surface charge of lipid vesicles and micelles

Singh, Yashveer and Gulyani, Akash and Bhattacharya, Santanu (2003) A new ratiometric fluorescence probe as strong sensor of surface charge of lipid vesicles and micelles. In: FEBS Letters, 541 (1-3). pp. 132-136.

PDF A_new_ratiometric_£uorescence_probe.pdf Restricted to Registered users only Download (140kB) | Request a copy

Abstract

We report on the ability of a new fluorescent probe, 4-(2-pyren-1-yl-vinyl) pyridine, 1, to respond to micelles and phospholipid vesicles of different surface charge. The probe gets incorporated into micellar and membranous assemblies and shows a large red-shift in the fluorescence emission maxima especially when the surface charge of the organized media is anionic. The effect on the photo-excitation of the probe is very clear and pronounced as it can be easily visualized. The sample color upon photo-excitation changes from blue to orange/red once the probe experiences negatively charged vesicular or micellar surfaces. These results make the probe molecule useful as a reporter for sensing electrostatic environment in biological membranes and related organized assemblies.

Item Type:	Journal Article
Publication:	FEBS Letters
Publisher:	Elsevier
Additional Information:	Copyright of this article belongs to Elsevier.
Keywords:	Membrane surface charge;Ratiometric probe;Pyren-1-yl-vinyl pyridine;Anionic surface;Phospholipid vesicle;Micelle
Department/Centre:	Division of Chemical Sciences > Organic Chemistry
Date Deposited:	25 Sep 2007
Last Modified:	19 Sep 2010 04:39
URI:	http://eprints.iisc.ac.in/id/eprint/11922

Actions (login required)

View Item