ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

Mammalian Brain Plasma Membranes: Isolation, Enzymatic, and Characterization

Krishnan, KS and Balaram, P (1976) Mammalian Brain Plasma Membranes: Isolation, Enzymatic, and Characterization. In: Experimental Cell Research, 101 . pp. 299-306.

[img] PDF
Restricted to Registered users only

Download (2MB) | Request a copy


Two variants of a simplified procedure for the isolation of plasma membrane fractions from monkey and rat brains, are described. The preparations showed marked enrichments in the marker enzymes, (Na+ - K+) adenosine triphosphatase, acetylcholinesterase, 5’-nucleotidase and adenylate cyclase. Lipid analysis and a protein electrophoretic pattern are presented. An enzymatic check has been made to assess for contamination by other cellular organelles. The amino acid composition of brain membrane proteins show a resemblance to the reported composition of erythrocyte ghost proteins but differ from myelin proteins.

Item Type: Journal Article
Publication: Experimental Cell Research
Publisher: Academic Press
Additional Information: Copyright for this article belongs to Academic Press
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 26 Jul 2004
Last Modified: 19 Sep 2010 04:14
URI: http://eprints.iisc.ac.in/id/eprint/1159

Actions (login required)

View Item View Item