Catechol dioxygenase is an insensitive reporter for transcription in Mycobacterium smegmatis

Saravanan, Matheshwaran and Elango, Kathirvel and Chandrashekaran, Siddamadappa and Anand, Narayanaswamy and Nagaraja, Valakunja (2003) Catechol dioxygenase is an insensitive reporter for transcription in Mycobacterium smegmatis. In: Current Science, 85 (2). pp. 188-191.

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Abstract

Although restriction enzymes are widely distributed in nature, many bacterial genera are yet to be explored for the presence of this important class of enzymes. We have purified and characterized a new type II restriction endonuclease, OfoI from a nonheterocyst cyanobacterium Oscillatoria foreaui. The recognition sequence has been determined by primer extension analysis. The purified enzyme OfoI recognizes and cleaves the palindromic hexanucleotide 5¢-C¯YCGRG-3¢, generating 5¢-protruding ends.

Item Type:	Journal Article
Publication:	Current Science
Publisher:	Indian Academy of Science
Keywords:	nonheterocystous cyanobacterium Oscillatoria foreaui
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	22 Jul 2004
Last Modified:	19 Sep 2010 04:14
URI:	http://eprints.iisc.ac.in/id/eprint/1124

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