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Perturbation of lipid structures by fluorescent probes

Krishnan, KS and Balaram, P (1975) Perturbation of lipid structures by fluorescent probes. In: FEBS Letters, 60 (2). pp. 419-422.

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The equilibrium fluorescent probe 1 -aniline-8-naphthalene sulfonate*, has found extensive use in studies of membrane structure. In an entertainingly written review, Radda and Vanderkooi have posed the question, ‘Can fluorescent probes tell us anything about membranes?’ [1]. A great deal of useful information has been derived from such probe studies. However the important question, ‘Do fluorescent probes perturb biomembranes?’ has not been conclusively answered. Indeed it has been suggested that probes can act as ‘molecular tumours’ and that unless proved otherwise a probe must be suspect of acting as a perturbant of the region it is monitoring [2]. In this communication, we report the use of the fluorescent hydrocarbon pyrene, to monitor structural changes, in lipids caused by ANS binding.

Item Type: Journal Article
Publication: FEBS Letters
Publisher: Elsevier
Additional Information: Copyright for this article belongs to Elsevier Science Ltd
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 03 Sep 2004
Last Modified: 19 Sep 2010 04:14
URI: http://eprints.iisc.ac.in/id/eprint/1122

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