DNA methylation in mycobacteria: Absence of methylation at GATC (Dam) and CCA/TGG (Dcm) sequences

Kirugaval, C and Hemavathy, I and Nagaraja, Valakunja (1995) DNA methylation in mycobacteria: Absence of methylation at GATC (Dam) and CCA/TGG (Dcm) sequences. In: FFMS Immunology and Medical Microbiology, 11 (4). pp. 291-296.

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Abstract

The presence of 6-methyladenine and 5-methylcytosine at Dam (GATC) and Dcm (CCA/TGG) sites in DNA of mycobacterial species was investigated using isoschizomer restriction enzymes. In all species examined, Dam and Dcm recognition sequences were not methylated indicating the absence of these methyltransferases. On the other hand, high performance liquid chromatographic analysis of genomic DNA from Mycobacterium smegmatis and Mycobacterium tuberculosis showed significant levels of 6-methyladenine and 5-methylcytosine suggesting the presence of DNA methyltransferases other than Dam and Dcm. Occurrence of methylation was also established by a sensitive genetic assay.

Item Type:	Journal Article
Publication:	FFMS Immunology and Medical Microbiology
Publisher:	Elsevier
Keywords:	Mycobacteria;DNA methylation;dam;dcm
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	06 Nov 2004
Last Modified:	19 Sep 2010 04:13
URI:	http://eprints.iisc.ac.in/id/eprint/1017

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