Seahorse assay for the mitochondrial respiration using Saccharomyces cerevisiae as a model system

Kumar, A and Waingankar, TP and D'Silva, P (2024) Seahorse assay for the mitochondrial respiration using Saccharomyces cerevisiae as a model system. In: Methods in Enzymology .

PDF met_enz_2024 - Published Version Restricted to Registered users only Download (1MB) | Request a copy

Abstract

Eukaryotic cells require energy to perform diverse cellular functions critical for survival. Mitochondria are multifunctional organelles that generate energy in the form of Adenosine triphosphate by oxidative phosphorylation, emphasizing their importance to eukaryotic cell viability. The ability of mitochondria to consume oxygen for respiration is a key parameter in assessing mitochondrial health. Therefore, developing new techniques to monitor mitochondrial respiration are crucial for advancing our understanding of organelle functioning. Recently, Seahorse technology has emerged as a valuable tool to analyze various aspects of mitochondrial bioenergetics. Although the Seahorse assay is well established in adherent cell lines and other model organisms, it remains challenging to employ it efficiently in yeast, a powerful genetic system for studying mitochondrial biology. In this chapter, we provide a comprehensive methodology for assessing oxygen consumption rate in baker's yeast using Seahorse. © 2024

Item Type:	Journal Article
Publication:	Methods in Enzymology
Publisher:	Academic Press Inc.
Additional Information:	The copyright for this article belongs to publisher.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	17 Sep 2024 11:21
Last Modified:	17 Sep 2024 11:21
URI:	http://eprints.iisc.ac.in/id/eprint/86167

Actions (login required)

View Item