Protective Role of Nanoceria-Infused Nanofibrous Scaffold toward Bone Tissue Regeneration with Senescent Cells

Nilawar, S and Yadav, P and Jain, N and Saini, DK and Chatterjee, K (2024) Protective Role of Nanoceria-Infused Nanofibrous Scaffold toward Bone Tissue Regeneration with Senescent Cells. In: Biomacromolecules, 25 (7). pp. 4074-4086.

PDF bio_2024.pdf - Published Version Restricted to Registered users only Download (9MB) | Request a copy

Abstract

The presence of oxidative stress in bone defects leads to delayed regeneration, especially in the aged population and patients receiving cancer treatment. This delay is attributed to the increased levels of reactive oxygen species (ROS) in these populations due to the accumulation of senescent cells. Tissue-engineered scaffolds are emerging as an alternative method to treat bone defects. In this study, we engineered tissue scaffolds tailored to modulate the adverse effects of oxidative stress and promote bone regeneration. We used polycaprolactone to fabricate nanofibrous mats by using electrospinning. We exploited the ROS-scavenging properties of cerium oxide nanoparticles to alleviate the high oxidative stress microenvironment caused by the presence of senescent cells. We characterized the nanofibers for their physical and mechanical properties and utilized an ionization-radiation-based model to induce senescence in bone cells. We demonstrate that the presence of ceria can modulate ROS levels, thereby reducing the level of senescence and promoting osteogenesis. Overall, this study demonstrates that ceria-infused nanofibrous scaffolds can be used for augmenting the osteogenic activity of senescent progenitor cells, which has important implications for engineering bone tissue scaffolds for patients with low regeneration capabilities. © 2024 American Chemical Society.

Item Type:	Journal Article
Publication:	Biomacromolecules
Publisher:	American Chemical Society
Additional Information:	The copyright for this article belongs to American Chemical Society.
Keywords:	Biomechanics; Bone; Cell engineering; Cells; Cytology; Defects; Nanofibers; Oxidative stress; Patient treatment; Scaffolds (biology); Tissue regeneration, Adverse effect; Bone defect; Bone regeneration; Bone tissue regeneration; Engineered tissue scaffolds; Nano-ceria; Nano-fibrous; Nanofibrous scaffolds; Reactive oxygen species; Tissue-engineered scaffolds, Cerium oxide
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy)
Date Deposited:	20 Aug 2024 12:43
Last Modified:	20 Aug 2024 12:43
URI:	http://eprints.iisc.ac.in/id/eprint/85482

Actions (login required)

View Item