Biochemical Characterization of Acyl-CoA: Lysophosphatidylcholine Acyltransferase (LPCAT) Enzyme from the Seeds of Salvia hispanica

Gopalam, R and Datey, A and Bijoor, S and Chakravortty, D and Tumaney, AW (2021) Biochemical Characterization of Acyl-CoA: Lysophosphatidylcholine Acyltransferase (LPCAT) Enzyme from the Seeds of Salvia hispanica. In: Molecular Biotechnology .

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Abstract

Salvia hispanica (chia) is the highest reported terrestrial plant source of alpha-linolenic acid (ALA, ~ 65), an �-3 polyunsaturated fatty acid with numerous health benefits. The molecular basis of high ALA accumulation in chia is yet to be understood. We have identified lysophosphatidylcholine acyltransferase (LPCAT) gene from the developing seed transcriptome data of chia and carried out its biochemical characterization through heterologous expression in Saccharomyces cerevisiae. Expression profiling showed that the enzyme was active throughout the seed development, indicating a pivotal role in oil biosynthesis. The enzyme could utilize both saturated and unsaturated lysophosphatidylcholine substrates at the same rate, to synthesize phosphatidylcholine (PC). The enzyme also exhibited lysophosphatidic acid acyltransferase (LPAAT) activity, by preferring lysophosphatidic acid substrate. Substrate specificity studies showed that the enzyme preferred both monounsaturated and polyunsaturated fatty acyl CoAs over saturated CoAs. This activity may play a key role in enriching the PC fraction with polyunsaturated fatty acids (PUFAs). PUFAs present on PC can be transferred to oil through the action of other acyltransferases. Our results describe a new LPCAT enzyme that can be used to biotechnologically alter oilseed crops to incorporate more PUFA in its seed oil. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

Item Type:	Journal Article
Publication:	Molecular Biotechnology
Publisher:	Humana Press Inc.
Additional Information:	The copyright for this article belongs to Humana Press Inc.
Keywords:	Biochemistry; Phospholipids; Polyunsaturated fatty acids; Substrates; Yeast, Alpha linolenic acids; Biochemical characterization; Heterologous expression; Lysophosphatidic acid; Lysophosphatidyl-choline; Omega-3 polyunsaturated fatty acids; Polyunsaturated fatty acids (PUFAs); Substrate specificity, Enzymes
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	30 Aug 2021 06:59
Last Modified:	30 Aug 2021 06:59
URI:	http://eprints.iisc.ac.in/id/eprint/69547

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