Kadam, NY and Behera, S and Kumar, S and Ghosh-Roy, A and Babu, K (2021) The g-protein-coupled receptor srx-97 is required for concentration-dependent sensing of benzaldehyde in caenorhabditis elegans. In: eNeuro, 8 (1). pp. 1-15.
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Abstract
The G-protein (heterotrimeric guanine nucleotide-binding protein)-coupled receptors (GPCRs) in the olfactory system function to sense the surrounding environment and respond to various odorants. The genes coding for olfactory receptors in Caenorhabditis elegans are larger in number in comparison to those in mammals, sug-gesting complexity in the receptor-odorant relationships. Recent studies have shown that the same odorant in different concentrations could act on multiple receptors in different neurons to induce attractive or repulsive responses. The ASH neurons are known to be responsible for responding to high concentrations of volatile odorants. Here, we characterize a new GPCR, SRX-97. We found that the srx-97 promoter drives expression specifically in the head ASH and tail PHB chemosensory neurons of C. elegans. Moreover, the SRX-97 protein localizes to the ciliary ends of the ASH neurons. Analysis of clustered regularly interspaced short palindromic repeats (CRISPR)-based deletion mutants of the srx-97 locus suggests that this gene is involved in recognition of high concentrations of benzaldehyde. This was further confirmed through rescue and neuronal ablation ex-periments. Our work brings novel insights into concentration-dependent receptor function in the olfactory sys-tem, and provides details of an additional molecule that helps the animal navigate its surroundings. © 2021 Kadam et al.
| Item Type: | Journal Article |
|---|---|
| Publication: | eNeuro |
| Publisher: | Society for Neuroscience |
| Additional Information: | The copyright of this article belongs to Society for Neuroscience |
| Department/Centre: | Division of Biological Sciences > Centre for Neuroscience |
| Date Deposited: | 15 Feb 2021 10:43 |
| Last Modified: | 15 Feb 2021 10:43 |
| URI: | http://eprints.iisc.ac.in/id/eprint/67963 |
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