Dual role for fungal-specific outer kinetochore proteins during cell cycle and development in Magnaporthe oryzae

Shah, Hiral and Rawat, Kanika and Ashar, Harsh and Patkar, Rajesh and Manjrekar, Johannes (2019) Dual role for fungal-specific outer kinetochore proteins during cell cycle and development in Magnaporthe oryzae. In: JOURNAL OF CELL SCIENCE, 132 (12).

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Abstract

The outer kinetochore DASH complex (also known as the Dam1 complex) ensures proper spindle structure and chromosome segregation. While DASH complex protein requirement diverges among different yeasts, its role in filamentous fungi has not yet been investigated. We studied the dynamics and role of middle (Mis12) and outer (Dam1 and Ask1) kinetochore proteins in the filamentous fungal pathogen, Magnaporthe oryzae, which undergoes multiple cell cyclelinked developmental transitions. While Mis12 was constitutively present in the nucleus, Dam1 and Ask1 were recruited only during mitosis. Although Dam1 was not required for viability, loss of its function (dam1 Delta) delayed mitotic progression, resulting in impaired conidial and hyphal development. Both Dam1 and Ask1 also localised to the hyphal tips, in the form of punctae oscillating back and forth from the growing ends, suggesting that Magnaporthe DASH complex proteins may play a non-canonical role in polarised growth during interphase, in addition to their function in nuclear segregation during mitosis. Impaired appressorial (infection structure) development and host penetration in the dam1. mutant suggest that fungus-specific Dam1 complex proteins could be an attractive target for a novel anti-fungal strategy.

Item Type:	Journal Article
Publication:	JOURNAL OF CELL SCIENCE
Publisher:	COMPANY BIOLOGISTS LTD
Additional Information:	Copyright of this article belongs to COMPANY BIOLOGISTS LTD
Keywords:	Kinetochore; Dam1 complex; DASH complex; Filamentous fungus; Magnaporthe; Rice blast fungus
Department/Centre:	Division of Biological Sciences > Centre for Ecological Sciences
Date Deposited:	15 Nov 2019 12:05
Last Modified:	15 Nov 2019 12:05
URI:	http://eprints.iisc.ac.in/id/eprint/63272

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