A NOVEL HIGH THROUGHPUT MICROFLUIDIC CELL TRANSIT ANALYZER USING AIR LIQUID INTERFACE AS LOCALIZED PRESSURE SENSOR

Koiesa, R S and Joshi, K R and Ancuithasuresh, G K and Sen, P (2018) A NOVEL HIGH THROUGHPUT MICROFLUIDIC CELL TRANSIT ANALYZER USING AIR LIQUID INTERFACE AS LOCALIZED PRESSURE SENSOR. In: 31st IEEE International Conference on Micro Electro Mechanical Systems (MEMS), JAN 21-25, 2018, Belfast, NORTH IRELAND, pp. 133-136.

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Abstract

We report a novel microfluidic platform that measures pressure drop during the passage of a single cell through a mechanical constriction for mechano-phenotyping of cell populations. It uses a precisely positioned Air-Liquid Interface as a Sensor (ALIaS) near the outlet of the constriction. When cells pass through the constriction they momentarily plug the inlet resulting in a drop in the hydrodynamic pressure at the outlet of the constriction. This change in pressure leads to motion of the air-liquid interface which can be correlated to entry and transit time of the cell passage. ALIaS facilitates accurate measurements of cell entry and transit time at high throughout (>200 cells/s). The platform can reliably quantify the variation in deformability in cell populations upon treatment with Glutaraldehyde. The system presents a potential for mechanobiological studies and can be made portable through electrical measurements of interface oscillations.

Item Type:	Conference Proceedings
Series.:	Proceedings IEEE Micro Electro Mechanical Systems
Publisher:	IEEE, 345 E 47TH ST, NEW YORK, NY 10017 USA
Additional Information:	Copyright of this article belong to IEEE, 345 E 47TH ST, NEW YORK, NY 10017 USA
Department/Centre:	Division of Mechanical Sciences > Mechanical Engineering Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering
Date Deposited:	05 Jul 2018 15:24
Last Modified:	05 Jul 2018 15:24
URI:	http://eprints.iisc.ac.in/id/eprint/60156

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