Influence of triacontanol and jasmonic acid on metabolomics during early stages of root induction in cultured tissue of tomato (Lycopersicon esculentum)

Soundararajan, Malini and Swamy, G Sivakumar and Gaonkar, Sumana Krishna and Deshmukh, Sudha (2018) Influence of triacontanol and jasmonic acid on metabolomics during early stages of root induction in cultured tissue of tomato (Lycopersicon esculentum). In: PLANT CELL TISSUE AND ORGAN CULTURE, 133 (1). pp. 147-157.

PDF Pla_Cel_Tis_Org_Cul_133-1_147_2018.pdf - Published Version Restricted to Registered users only Download (3MB) | Request a copy

Abstract

Influence of n-triacontanol (TRIA) and jasmonic acid (JA) on metabolic profiling during root morphogenesis was studied in Lycopersicon esculentum (cv. PKM-1). Proton nuclear magnetic resonance (H-1 NMR) based metabolomics was employed to investigate the variations in metabolic profile. Chenomx NMR suite v.8.1 was used to identify and quantify metabolites based on their respective signature spectra. The levels of 47 metabolites were monitored for 72 h at specific time intervals (0, 3, 6, 9, 12, 24, 36, 48 and 72 h). Principal component analysis was performed to determine the variations in the metabolic profile between control and treatments during in vitro rhizogenesis. TRIA was observed to promote early root emergence (24 h) and also influence the metabolic variation during rhizogenesis between 9 and 24 h post exposure. Compounds such as IAA, ATP, NADPH, UDP-N-acetylglucosamine and gallate predominated at 9 h. Unlike TRIA, JA was unable to promote an early root induction. However, it influenced the synthesis of a relatively higher concentration of IAA at 6 h when compared to ATP, NADPH and trigonelline at 9 h. In the presence of both TRIA and JA (TRIA + JA), significant changes in the metabolic profiles were observed 24 h post exposure and the rooting was observed only after 72 h. The study suggests that TRIA may accelerate in vitro rhizogenesis of cultured tomato tissues by mainly increasing the synthesis of other growth promoting metabolites. But in the presence of JA, TRIA's effect appears to be reduced.

Item Type:	Journal Article
Publication:	PLANT CELL TISSUE AND ORGAN CULTURE
Publisher:	SPRINGER, VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
Additional Information:	Copy right for the article belong to SPRINGER, VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
Department/Centre:	Division of Chemical Sciences > NMR Research Centre (Formerly Sophisticated Instruments Facility)
Date Deposited:	02 Apr 2018 19:59
Last Modified:	02 Apr 2018 19:59
URI:	http://eprints.iisc.ac.in/id/eprint/59434

Actions (login required)

View Item