Functional characterization of recombinant major envelope protein (rB2L) of orf virus

Yogisharadhya, Revanaiah and Kumar, Amit and Ramappa, Raghavendra and Venkatesan, Gnanavel and Bhanuprakash, Veerakyathappa and Shivachandra, Sathish Bhadravati (2017) Functional characterization of recombinant major envelope protein (rB2L) of orf virus. In: ARCHIVES OF VIROLOGY, 162 (4). pp. 953-962.

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Abstract

Orf, or contagious ecthyma, a highly contagious transboundary disease of sheep and goats, is caused by a double-stranded DNA virus (ORFV) belonging to the genus Parapoxvirus of the family Poxviridae. The ORFV genome encodes the major envelope proteins B2L and F1L, which have been found to be highly immunogenic and have multiple functional characteristics. In order to investigate the functional properties of the B2L protein, in this study, the B2L gene of ORFV strain 59/05, encoding recombinant mature B2L (aa M-1-D-334), was produced as a fusion protein in Escherichia coli. The functional characteristics of purified rB2L fusion protein (similar to 60 kDa) were evaluated in vivo and in vitro, showing that this protein had lipase and immunomodulatory activities. Immunization trials involving laboratory animals (mice, rabbits and guinea pigs) using either constant or graded doses of rB2L fusion protein with or without adjuvants (FCA, alum) as well as coadministration with candidate rErns-Ag protein of classical swine fever virus (CSFV) indicated that the rB2L protein is immunogenic and has immunomodulatory properties. This study shows the potential utility of the rB2L protein as a safe and novel adjuvant in veterinary vaccine formulations.

Item Type:	Journal Article
Publication:	ARCHIVES OF VIROLOGY
Publisher:	SPRINGER WIEN, SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA
Additional Information:	Copy right for this article belongs to the SPRINGER WIEN, SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	20 May 2017 04:34
Last Modified:	12 Oct 2018 06:47
URI:	http://eprints.iisc.ac.in/id/eprint/56863

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