Platinum(II) Complexes of Curcumin Showing Photocytotoxicity in Visible Light

Mitra, Koushambi and Gautam, Srishti and Kondaiah, Paturu and Chakravarty, Akhil R (2017) Platinum(II) Complexes of Curcumin Showing Photocytotoxicity in Visible Light. In: EUROPEAN JOURNAL OF INORGANIC CHEMISTRY (12, SI). pp. 1753-1763.

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Abstract

Three platinum(II) complexes of curcumin, Pt(NH3)(2)( cur)](NO3) (1), Pt(en)(cur)](NO3) (2) and Pt(dach)(cur)](NO3) (3), where Hcu is curcumin, en is ethylenediamine and dach is 1R,2R-(-)-1,2-diaminocyclohexane, were synthesized, characterized and their photocytotoxic properties were explored. The acetylacetone (Hacac) analogue of 1, Pt(NH3)(2)(acac)] NO3 (4), was prepared to investigate the role of photoactive curcumin. The crystal structure of Pt(NH3)(2)(acac)] CF3SO3 (4a) revealed square-planar geometry of the platinum(II) centre. Complexes 1-3 displayed an intense absorption band at 450 nm (epsilon = 22500 M-1 cm-1). The curcumin in metal-bound form showed improved stability in 10 % DMSO/buffer solution at 37 degrees C. The complexes released curcumin upon irradiation with visible light (400-700 nm) as evident from the increment in emission intensity. The complexes formed Pt-DNA covalent adducts when treated with calf thymus (ct)-DNA and exposed to light. The platinum-curcumin conjugates demonstrated photocytotoxicity in cancer cells giving IC50 values of 15-45 mu M (400-700 nm, 10 J cm(-2)) with no apparent activity in the dark (IC50 > 200 mu M). Cytoplasmic localization and reactive oxygen species (ROS)mediated apoptotic cell death in visible light was observed for the curcumin complexes.

Item Type:	Journal Article
Publication:	EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
Additional Information:	Copy right for this article belongs to the WILEY-V C H VERLAG GMBH, POSTFACH 101161, 69451 WEINHEIM, GERMANY
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics Division of Chemical Sciences > Inorganic & Physical Chemistry
Date Deposited:	26 Apr 2017 07:23
Last Modified:	26 Apr 2017 07:23
URI:	http://eprints.iisc.ac.in/id/eprint/56643

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