Aza-heterocyclic Receptors for Direct Electron Transfer Hemoglobin Biosensor

Kumar, Vinay and Kashyap, Nikhila D M and Hebbar, Suraj and Swetha, R and Prasad, Sujay and Kamala, T and Srikanta, S S and Krishnaswamy, P R and Bhat, Navakanta (2017) Aza-heterocyclic Receptors for Direct Electron Transfer Hemoglobin Biosensor. In: SCIENTIFIC REPORTS, 7 .

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Abstract

Direct Electron Transfer biosensors, facilitating direct communication between the biomolecule of interest and electrode surface, are preferable compared to enzymatic and mediator based sensors. Although hemoglobin (Hb) contains four redox active iron centres, direct detection is not possible due to inaccessibility of iron centres and formation of dimers, blocking electron transfer. Through the coordination of iron with aza-heterocyclic receptors-pyridine and imidazole-we report a cost effective, highly sensitive and simple electrochemical Hb sensor using cyclic voltammetry and chronoamperometry. The receptor can be either in the form of liquid micro-droplet mixed with blood or dry chemistry embedded in paper membrane on top of screen printed carbon electrodes. We demonstrate excellent linearity and robustness against interference using clinical samples. A truly point of care technology is demonstrated by integrating disposable test strips with handheld reader, enabling finger prick to result in less than a minute.

Item Type:	Journal Article
Publication:	SCIENTIFIC REPORTS
Publisher:	NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
Additional Information:	Copy right for this article belongs to the NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
Department/Centre:	Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering
Date Deposited:	09 Mar 2017 06:00
Last Modified:	09 Mar 2017 06:00
URI:	http://eprints.iisc.ac.in/id/eprint/56349

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