Specific detection of the cleavage activity of mycobacterial enzymes using a quantum dot based DNA nanosensor

Jepsen, Morten Leth and Harmsen, Charlotte and Godbole, Adwait Anand and Nagaraja, Valakunja and Knudsen, Birgitta R and Ho, Yi-Ping (2016) Specific detection of the cleavage activity of mycobacterial enzymes using a quantum dot based DNA nanosensor. In: NANOSCALE, 8 (1). pp. 358-364.

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Abstract

We present a quantum dot based DNA nanosensor specifically targeting the cleavage step in the reaction cycle of the essential DNA-modifying enzyme, mycobacterial topoisomerase I. The design takes advantages of the unique photophysical properties of quantum dots to generate visible fluorescence recovery upon specific cleavage by mycobacterial topoisomerase I. This report, for the first time, demonstrates the possibility to quantify the cleavage activity of the mycobacterial enzyme without the pre-processing sample purification or post-processing signal amplification. The cleavage induced signal response has also proven reliable in biological matrices, such as whole cell extracts prepared from Escherichia coli and human Caco-2 cells. It is expected that the assay may contribute to the clinical diagnostics of bacterial diseases, as well as the evaluation of treatment outcomes.

Item Type:	Journal Article
Publication:	NANOSCALE
Publisher:	ROYAL SOC CHEMISTRY
Additional Information:	Copy right for this article belongs to the ROYAL SOC CHEMISTRY, THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	20 Jan 2016 05:34
Last Modified:	20 Jan 2016 05:34
URI:	http://eprints.iisc.ac.in/id/eprint/53139

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