Singh, Aditya and Kumar, Suraj and Singh, Vikram Pal and Balaji, J (2013) Optical microscopy methods for understanding learning and memory. In: CURRENT SCIENCE, 105 (11). pp. 1537-1548.
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Abstract
Structural dynamics of dendritic spines is one of the key correlative measures of synaptic plasticity for encoding short-term and long-term memory. Optical studies of structural changes in brain tissue using confocal microscopy face difficulties of scattering. This results in low signal-to-noise ratio and thus limiting the imaging depth to few tens of microns. Multiphoton microscopy (MpM) overcomes this limitation by using low-energy photons to cause localized excitation and achieve high resolution in all three dimensions. Multiple low-energy photons with longer wavelengths minimize scattering and allow access to deeper brain regions at several hundred microns. In this article, we provide a basic understanding of the physical phenomena that give MpM an edge over conventional microscopy. Further, we highlight a few of the key studies in the field of learning and memory which would not have been possible without the advent of MpM.
| Item Type: | Journal Article |
|---|---|
| Publication: | CURRENT SCIENCE |
| Publisher: | INDIAN ACAD SCIENCES |
| Additional Information: | Copyright for this article belongs to the INDIAN ACAD SCIENCES, INDIA |
| Keywords: | Learning; memory; optical microscopy; two-photon excitation |
| Department/Centre: | Division of Biological Sciences > Centre for Neuroscience |
| Date Deposited: | 17 Feb 2014 05:34 |
| Last Modified: | 17 Feb 2014 05:34 |
| URI: | http://eprints.iisc.ac.in/id/eprint/48380 |
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