Effect of myonuclear number and mitochondrial fusion on Drosophila indirect flight muscle organization and size

Rai, Mamta and Nongthomba, Upendra (2013) Effect of myonuclear number and mitochondrial fusion on Drosophila indirect flight muscle organization and size. In: EXPERIMENTAL CELL RESEARCH, 319 (17). pp. 2566-2577.

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Abstract

Mechanisms involved in establishing the organization and numbers of fibres in a muscle are not completely understood. During Drosophila indirect flight muscle (IFM) formation, muscle growth is achieved by both incorporating hundreds of nuclei, and hypertrophy. As a result, IFMs provide a good model with which to understand the mechanisms that govern overall muscle organization and growth. We present a detailed analysis of the organization of dorsal longitudinal muscles (DLMs), a subset of the IFMs. We show that each DLM is similar to a vertebrate fascicle and consists of multiple muscle fibres. However, increased fascicle size does not necessarily change the number of constituent fibres, but does increase the number of myofibrils packed within the fibres. We also find that altering the number of myoblasts available for fusion changes DLM fascicle size and fibres are loosely packed with myofibrils. Additionally, we show that knock down of genes required for mitochondrial fusion causes a severe reduction in the size of DLM fascicles and fibres. Our results establish the organization levels of DLMs and highlight the importance of the appropriate number of nuclei and mitochondrial fusion in determining the overall organization, growth and size of DLMs. (C) 2013 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Publication:	EXPERIMENTAL CELL RESEARCH
Publisher:	ELSEVIER INC
Additional Information:	copyright for this article belongs to ELSEVIER INC
Keywords:	Mitochondrial fusion; Myoblast; Myonuclear number; Muscle organization; Indirect flight muscles; Drosophila
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	26 Nov 2013 15:17
Last Modified:	26 Nov 2013 15:17
URI:	http://eprints.iisc.ac.in/id/eprint/47788

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