A new type II restriction endonuclease, OfoI from nonheterocystous cyanobacterium Oscillatoria foreaui

Saravanan, Matheshwaran and Elango, Kathirvel and Chandrashekaran, Siddamadappa and Anand, Narayanaswamy and Nagaraja, Valakunja (2003) A new type II restriction endonuclease, OfoI from nonheterocystous cyanobacterium Oscillatoria foreaui. In: Current Science (Bangalore), 85 (2). pp. 188-191.

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Abstract

Although restriction enzymes are widely distributed in nature, many bacterial genera are yet to be explored for the presence of this important class of enzymes. We have purified and characterized a new type II restriction endonuclease, OfoI from a nonheterocyst cyanobacterium Oscillatoria foreaui. The recognition sequence has been determined by primer extension analysis. The purified enzyme OfoI recognizes and cleaves the palindromic hexanucleotide 5'-Cdown arrowYCGRG-3', generating 5'-protruding ends.

Item Type:	Journal Article
Publication:	Current Science (Bangalore)
Publisher:	Indian Academy of Sciences
Additional Information:	Copyright of this article belongs to Indian Academy of Sciences.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	04 Aug 2011 06:55
Last Modified:	04 Aug 2011 06:55
URI:	http://eprints.iisc.ac.in/id/eprint/39753

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