Crystallization and preliminary X-ray studies of a galactose-specific lectin from the seeds of Spatholobus parviflorus

Geethanandan, K and Abhilash, Joseph and Bharath, SR and Sadasivan, C and Haridas, M (2011) Crystallization and preliminary X-ray studies of a galactose-specific lectin from the seeds of Spatholobus parviflorus. In: Acta Crystallographica Section F, 67 (Part 6). pp. 700-702.

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Abstract

A galactose-specific seed lectin was purified from the legume Spatholobus parviflorus and crystallized using the hanging-drop vapour-diffusion technique. The crystals belonged to space group P1, with unit-cell parameters a = 60.998, b = 60.792, c = 78.179 angstrom, alpha = 101.32, beta = 91.38, gamma = 104.32 degrees. X-ray diffraction data were collected under cryoconditions (100 K) to a resolution of 2.04 angstrom using a MAR image-plate detector system mounted on a rotating-anode X-ray (Cu K alpha) generator. Molecular replacement using legume-lectin coordinates as a search model gave a tetrameric structure.

Item Type:	Journal Article
Publication:	Acta Crystallographica Section F
Publisher:	John Wiley and Sons
Additional Information:	Copyright of this article belongs to John Wiley and Sons.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	22 Jun 2011 09:50
Last Modified:	22 Jun 2011 09:50
URI:	http://eprints.iisc.ac.in/id/eprint/38442

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