Needleless Vaccine Delivery Using Micro-Shock Waves

Jagadeesh, Gopalan and Prakash, Divya G and Rakesh, SG and Allam, Uday Sankar and Krishna, Gopala M and Eswarappa, Sandeepa M and Chakravortty, Dipshikha (2011) Needleless Vaccine Delivery Using Micro-Shock Waves. In: Clinical and Vaccine Immunology, 18 (4). pp. 539-545.

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Abstract

Shock waves are one of the most efficient mechanisms of energy dissipation observed in nature. In this study, utilizing the instantaneous mechanical impulse generated behind a micro-shock wave during a controlled explosion, a novel nonintrusive needleless vaccine delivery system has been developed. It is well-known that antigens in the epidermis are efficiently presented by resident Langerhans cells, eliciting the requisite immune response, making them a good target for vaccine delivery. Unfortunately, needle-free devices for epidermal delivery have inherent problems from the perspective of the safety and comfort of the patient. The penetration depth of less than 100 mu m in the skin can elicit higher immune response without any pain. Here we show the efficient utilization of our needleless device (that uses micro-shock waves) for vaccination. The production of liquid jet was confirmed by high-speed microscopy, and the penetration in acrylamide gel and mouse skin was observed by confocal microscopy. Salmonella enterica serovar Typhimurium vaccine strain pmrG-HM-D (DV-STM-07) was delivered using our device in the murine salmonellosis model, and the effectiveness of the delivery system for vaccination was compared with other routes of vaccination. Vaccination using our device elicits better protection and an IgG response even at a lower vaccine dose (10-fold less) compared to other routes of vaccination. We anticipate that our novel method can be utilized for effective, cheap, and safe vaccination in the near future.

Item Type:	Journal Article
Publication:	Clinical and Vaccine Immunology
Publisher:	American Society for Microbiology
Additional Information:	Copyright of this article belongs to American Society for Microbiology.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology Division of Mechanical Sciences > Aerospace Engineering(Formerly Aeronautical Engineering)
Date Deposited:	26 Apr 2011 10:08
Last Modified:	26 Apr 2011 10:08
URI:	http://eprints.iisc.ac.in/id/eprint/37157

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