Sailaja, G and Nayak, R and Antony, A (1996) Azidothymidine induces apoptosis in mouse myeloma cell line Sp2/0. In: Biochemical Pharmacology, 52 (6). pp. 857-862.
PDF
Azidothymidine_Induces.pdf - Published Version Restricted to Registered users only Download (806kB) | Request a copy |
Abstract
Azidothymidine (AZT), which has been extensively used as an antiviral agent in the treatment of AIDS, showed strong inhibition of growth of Sp2/0 cells in vitro. AZT-treated cells showed a decrease in viability in a dose-dependent manner. AZT specifically induced typical apoptotic cell death with DNA double-strand cleavage and subsequent formation of apoptotic bodies. The induction of DNA double-strand cleavage into the oligonucleosomal ladder by AZT was protected in the presence of thymidine or uridine. An increase in endonuclease activity from nuclear extract of AZT-treated cells was observed. The enzyme activity was found to be Ca2+- and Mg2+-dependent and was inhibited by zinc acetate. A marked enhancement of PARP activity was observed in AZT-treated cells. These observations show that AZT can trigger both morphological and biochemical changes typical of apoptosis in the mouse myeloma cell line Sp2/0.
Item Type: | Journal Article |
---|---|
Publication: | Biochemical Pharmacology |
Publisher: | Elsevier science |
Additional Information: | Copyright of this article belongs to Elsevier science. |
Keywords: | azidothymidine;apoptosis;endonuclease;poly(ADP-ribose) polymerase |
Department/Centre: | Division of Biological Sciences > Microbiology & Cell Biology |
Date Deposited: | 21 Apr 2011 05:46 |
Last Modified: | 21 Apr 2011 05:46 |
URI: | http://eprints.iisc.ac.in/id/eprint/37045 |
Actions (login required)
View Item |