Characterization of oxygen free radicals generated during vanadate-stimulated NADH oxidation

Kalyani, P and Vijaya, S and Ramasarma, T (1992) Characterization of oxygen free radicals generated during vanadate-stimulated NADH oxidation. In: Molecular and Cellular Biochemistry, 111 (1-2). pp. 33-40.

PDF Characterization.pdf - Published Version Restricted to Registered users only Download (621kB) | Request a copy

Abstract

The oxidation of NADH and accompanying reduction of oxygen to H2O2 stimulated by polyvanadate was markedly inhibited by SOD and cytochrome c. The presence of decavanadate, the polymeric form, is necessary for obtaining the microsomal enzyme-catalyzed activity. The accompanying activity of reduction of cytochrome c was found to be SOD-insensitive and therefore does not represent superoxide formation. The reduction of cytochrome c by vanadyl sulfate was also SOD-insensitive. In the presence of H2O2 all the forms of vanadate were able to oxidize reduced cytochrome c, which was sensitive to mannitol, tris and also catalase, indicating H202-dependent generation of hydroxyl radicals. Using ESR and spin trapping technique only hydroxyl radicals, but not superoxide anion radicals, were detected during polyvanadate-dependent NADH oxidation.

Item Type:	Journal Article
Publication:	Molecular and Cellular Biochemistry
Publisher:	Springer
Additional Information:	Copyright of this article belongs to Springer.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	25 Nov 2011 07:25
Last Modified:	25 Nov 2011 07:25
URI:	http://eprints.iisc.ac.in/id/eprint/35119

Actions (login required)

View Item