Cationic Amino Acid Transporters and Salmonella Typhimurium ArgT Collectively Regulate Arginine Availability towards Intracellular Salmonella Growth

Das, Priyanka and Lahiri, Amit and Lahiri, Ayan and Sen, Minakshi and Iyer, Namrata and Kapoor, Nisha and Balaji, Kithiganahalli Narayanaswamy and Chakravortty, Dipshikha (2010) Cationic Amino Acid Transporters and Salmonella Typhimurium ArgT Collectively Regulate Arginine Availability towards Intracellular Salmonella Growth. In: PLos One, 5 (11).

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Abstract

Cationic amino acid transporters (mCAT1 and mCAT2B) regulate the arginine availability in macrophages. How in the infected cell a pathogen can alter the arginine metabolism of the host remains to be understood. We reveal here a novel mechanism by which Salmonella exploit mCAT1 and mCAT2B to acquire host arginine towards its own intracellular growth within antigen presenting cells. We demonstrate that Salmonella infected bone marrow derived macrophages and dendritic cells show enhanced arginine uptake and increased expression of mCAT1 and mCAT2B. We show that the mCAT1 transporter is in close proximity to Salmonella containing vacuole (SCV) specifically by live intracellular Salmonella in order to access the macrophage cytosolic arginine pool. Further, Lysosome associated membrane protein 1, a marker of SCV, also was found to colocalize with mCAT1 in the Salmonella infected cell. The intra vacuolar Salmonella then acquire the host arginine via its own arginine transporter, ArgT for growth. The argT knockout strain was unable to acquire host arginine and was attenuated in growth in both macrophages and in mice model of infection. Together, these data reveal survival strategies by which virulent Salmonella adapt to the harsh conditions prevailing in the infected host cells.

Item Type:	Journal Article
Publication:	PLos One
Publisher:	Public Library of Science
Additional Information:	This article is licensed under a Creative Commons Attribution License.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	27 Dec 2010 07:30
Last Modified:	27 Dec 2010 07:35
URI:	http://eprints.iisc.ac.in/id/eprint/34598

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