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Differential binding of peanut agglutinin with lipopolysaccharide of homologous and heterologous Rhizobium

Maitia, TK and Podder, SK (1989) Differential binding of peanut agglutinin with lipopolysaccharide of homologous and heterologous Rhizobium. In: FEMS Microbiology Letters, 65 (3). pp. 279-283.

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Official URL: http://www3.interscience.wiley.com/journal/1194417...

Abstract

To establish the crucial role of lipopolysaccharide in the initial recognition event of symbiotic peanut-Rhizobium system the ability of various surface polysaccharides isolated from Bradyrhizobium arachis to inhibit the precipitin reaction between peanut agglutinin and asialoganglioside: deoxycholate (1:1) micelles was estimated. It was compared with that of nonsymbiotic systems e.g. Bradyrhizobium japonicum, Bradyrhizobium ciceris and Escherichia coli. Peanut agglutinin was found to interact more strongly with the lipopolysaccharide of Bradyrhizobium arachis than the exopolysaccharide or capsular polysaccharide. The inhibitory capacity of lipopolysaccharides from homologous and heterologous Bradyrhizobium as measured in terms of the concentration necessary for 50 percent inhibition of precipitin reaction were 1428, 500, 410, and 277 times less than that of lactose for Bradyrhizobium arachis, B. japonicum, B. ciceris and Escherichia coli, respectively. These results support that host lectin peanut agglutinin can recognize homologous Bradyrhizobium lipopolysaccharide by virtue of its binding specificity of higher magnitude.

Item Type: Journal Article
Publication: FEMS Microbiology Letters
Publisher: John Wiley and Sons
Additional Information: Copyright of this article belongs to John Wiley and Sons.
Keywords: Asialoganglioside;Bradyrhizobium japonicum;Bradyrhizobium spp;Escherichia coli;Lipopolysaccharide;Micelle;Peanut agglutinin;Precipitin reaction.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 20 Jul 2010 09:27
Last Modified: 12 Oct 2010 06:40
URI: http://eprints.iisc.ac.in/id/eprint/30357

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