Murthy, GS and Srilatha, NS (1996) Mapping of assembled epitopes with microgram quantities of antigen: Identification of an epitope at the receptor binding region of human follicle stimulating hormone. In: Current Science, 70 (11). pp. 1019-1022.
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Identification of epitopes by modification studies has been reported by us recently. The method requires milligram quantities of antigen and since several proteins are not available in large quantities they are not amenable for such an investigation. One such protein is human follicle stimulating hormone (hFSH) whose mapping of epitopes is of importance in reproductive biology. Here we report a method that uses microgram quantities of hFSH to map a beta-specific epitope located at the receptor binding region. This identification has also been validated by the chemical modification method using heterologous antigen ovine follicle stimulating hormone (oFSH).
| Item Type: | Journal Article |
|---|---|
| Publication: | Current Science |
| Publisher: | Indian Academy of Sciences |
| Additional Information: | Copyright of this article belongs to Indian Academy of Sciences. |
| Department/Centre: | Division of Biological Sciences > Molecular Reproduction, Development & Genetics |
| Date Deposited: | 22 Jan 2010 06:36 |
| Last Modified: | 28 Feb 2010 13:02 |
| URI: | http://eprints.iisc.ac.in/id/eprint/24730 |
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