RNA polymerase activity in isolated nuclei of Nicotiana sanderae callus: Characteristics and modulation during differentiation

Asha, PK and Shaila, MS and Vaidyanathan, CS and Ramakrishnan, TV (1983) RNA polymerase activity in isolated nuclei of Nicotiana sanderae callus: Characteristics and modulation during differentiation. In: journal of Bioscience, 5 (4). pp. 347-353.

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Abstract

Isolated nuclei from differentiating cultures of Nicotiana sanderae showed increased levels of RNA polymerase activity as compared to the nuclei from callus cultures. The RNA synthetic activity was dependent on nucleotide triphosphates and Mg2+ and was destroyed by RNase. Maximum activity was obtained in the presence of 50 mM (NH4)2 SO4 and α-amanitin inhibited 40% and 55% of the activity in the nuclei from callus and differentiating tissue respectively. The nuclei from differentiating tissue elicited a 3-fold increase in RNA polymerase I and a 4-fold augmentation in RNA polymerase II activities.

Item Type:	Journal Article
Publication:	journal of Bioscience
Publisher:	Indian academy of sciences
Additional Information:	Copyright for this article belongs to Indian academy of sciences.
Keywords:	Nicotiana sanderae; RNA polymerase; phytohormones; differentiation.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	20 Jan 2010 05:49
Last Modified:	19 Sep 2010 05:47
URI:	http://eprints.iisc.ac.in/id/eprint/23694

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