Tubulin synthesis and auxin-induced root initiation in phaseolus

Kantharaj, GR and Mahadevan, S and Padmanabhan, G (1985) Tubulin synthesis and auxin-induced root initiation in phaseolus. In: Phytochemistry, 24 (1). pp. 23-27.

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Abstract

The auxin-induced formation of roots in the hypocotyls of Phaseolus vulgaris can be prevented by treatment with actinomycin D, colchicine or cytochalasin B if applied within 40 hr of initiation. Shortly after auxin pretreatment, there is an increase in translatable messenger RNA activity. Analysis of the labelled cell-free products indicate, among other changes, a striking increase in a protein co-migrating with tubulin, in the case of RNA isolated from indolebutyric acid (IBA) pretreated hypocotyls. An increase in tubulin content in vivo can also be demonstrated on the basis of SDS-polyacrylamide gel analysis of membrane proteins and functional assays for tubulin polymerization. An increase in the synthesis of tubulin in vivo can also be demonstrated after IBA pretreatment. In addition, the auxin is also able to promote tubulin polymerization when added in vitro. It is suggested that tubulin synthesis and microtubule assembly are early events in auxin-mediated root differentiation.

Item Type:	Journal Article
Publication:	Phytochemistry
Publisher:	Elsevier Science
Additional Information:	copyright of this article belongs to Elsevier Science
Keywords:	Phaseolus vulgaris; Leguminosae; french bean; hypocotyls; auxin; roots and tubulin.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	09 Nov 2009 08:24
Last Modified:	19 Sep 2010 05:41
URI:	http://eprints.iisc.ac.in/id/eprint/22294

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