Purification and partial characterization of microsomal cytochrome b555 from the higher plant Catharanthus-Roseus

Madyastha, K Madhava and Krishnamachary, N (1986) Purification and partial characterization of microsomal cytochrome b555 from the higher plant Catharanthus-Roseus. In: Biochemical and Biophysical Research Communications, 136 (2). 570 -576.

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Abstract

Microsomal b-type hemoprotein designated, cytochrome b555 of C-Roseus seedlings was solubilized using detergents and purified by a combination of ion exchange chromatography and gel filtration to a specific content of 18.5 nmol per mg of protein. The purified cytochrome b555 was homogeneous and estimated to have an apparent molecular weight of 16500 on SDS-PAGE. The absorption spectrum of the reduced form has major peaks at 424, 525 and 555 nm. The α-band of the reduced form is asymmetric with a pronounced shoulder at 559 nm. The spectrum of the pyridine ferrohemochrome shows absorption peaks at 557, 524 and 418 nm indicating that the cytochrome has protoheme prosthetic group. The purified cytochrome is autoxidizable and does not combine with carbon monoxide, azide or cyanide. It is reducible by NADH in the presence of NADH-cytochrome b555 reductase partially purified from C-Roseus microsomes.

Item Type:	Journal Article
Publication:	Biochemical and Biophysical Research Communications
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Department/Centre:	Division of Chemical Sciences > Organic Chemistry
Date Deposited:	01 Feb 2010 07:38
Last Modified:	19 Sep 2010 05:40
URI:	http://eprints.iisc.ac.in/id/eprint/22115

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