Parrack, PK and Datta, S and Sasisekharan, V (1984) A detailed study of Li-DNA fibres at various salt concentrations reveals a non-helical B-DNA and a possible similarity of solution and solid state structures. In: Journal Of Biomolecular Structure & Dynamics, 2 (1). pp. 149-157.
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A thorough investigation of salt concentration dependence of lithium DNA fibres is made using X-ray diffraction. While for low salt the C-form pattern is obtained, crystalline B-type diffraction patterns result on increasing the salt concentration. The salt content in the gel (from which fibres are drawn) is estimated by equilibrium dialysis using the Donnan equilibrium principle. The salt range giving the best crystalline B pattern is determined. It is found that in this range meridional reflections occur on the fourth and sixth layer lines. In addition, the tenth layer meridian is absent at a particular salt concentration. These results strongly suggest the presence of non-helical features in the DNA molecule. Preliminary analysis of the diffraction patterns indicates a structural variability within the B-form itself. Further, the possibility of the structural parameters of DNA being similar in solid state and in solution is discussed.
| Item Type: | Journal Article |
|---|---|
| Publication: | Journal Of Biomolecular Structure & Dynamics |
| Publisher: | Adenine Presses |
| Additional Information: | Copyright of this article belongs to Adenine Presses. |
| Department/Centre: | Division of Biological Sciences > Molecular Biophysics Unit |
| Date Deposited: | 10 Aug 2009 09:39 |
| Last Modified: | 10 Aug 2009 09:39 |
| URI: | http://eprints.iisc.ac.in/id/eprint/22026 |
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