Antigenic similarities among biotin-binding proteins

Subramanian, N and Subramanian, Sarada and Adiga, Radhakantha P (1996) Antigenic similarities among biotin-binding proteins. In: Biochemistry and Molecular Biology International, 40 (04). pp. 663-670.

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Abstract

Using polyclonal antisera to avidin, streptavidin, and biotin binding protein-II (BBP-II), the extents of immunological cross-reactivities shared by this family of biotin-binders were investigated. In Western blot analysis, varying degrees of cross-reactivities among these proteins depending on the antisera used was observed. Using liquid phase radioimmunoassay, evidence for the existence of common antigenic determinants shared by all the biotin-binders was deduced. Antibodies directed against such epitopes inhibited the binding of [H-3]biotin to as well as displace the bound ligand from all the three high affinity biotin-binders in a dose dependent manner

Item Type:	Journal Article
Publication:	Biochemistry and Molecular Biology International
Publisher:	Academic Press
Additional Information:	Copyright of this article belongs to Academic Press.
Keywords:	Biotin-binders;antibodies;ligand-binding;inhibition.
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	26 Feb 2009 07:18
Last Modified:	01 Mar 2012 07:13
URI:	http://eprints.iisc.ac.in/id/eprint/19080

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