Identification of a novel hydroxyproline-rich glycoprotein as the major allergen in Parthenium pollen

Gupta, Neetu and Martin, Brian M and Metcalfe, Dean D and Rao, Subba PV (1996) Identification of a novel hydroxyproline-rich glycoprotein as the major allergen in Parthenium pollen. In: Journal Of Allergy And Clinical Immunology, 98 (05). pp. 903-912.

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Abstract

Background: The airborne pollen of the Compositae weed, Parthenium hysterophorus, is a major cause of allergic rhinitis in the Indian subcontinent and in certain parts of the southern United States and western Australia. Earlier studies have identified a 31 kd protein as the major allergen in Parthenium pollen. Objective: This study was undertaken to carry out the purification, immunochemical characterization, sequencing and epitope analysis of this major allergen, designated as Pal h 1. Methods: The IgE-binding activity of the allergen was evaluated by immunoblot and inhibition ELISAs. Pronase digestion, periodate oxidation, and chemical deglycosylation were performed to determine the role of peptide and carbohydrate components of the allergen-in IgE binding. Results: The data provide evidence for the involvement of carbohydrate moieties on Par h 1 in its IgE-binding ability. The N-terminal 91 amino acid sequence of Par h 1 shows 81% identity with a protein from sunflower anther, and the hydroxyproline-rich region of Par h 1 is 30% to 40% identical to similar stretches in extensins, a class of hydroxyproline-rich cell wall glycoproteins from different plant species. IgE antibodies in the sera of individuals allergic to Parthenium cross-reacted with a 50 kd hydroxproline-arabinose-rich extensin precursor from potato tuber, and this binding was periodate-sensitive. Conclusions: It appears that a group of soluble plant glycoproteins, which are related td the ubiquitous extensins, have certain car carbohydrate-containing IgE-binding epitopes that may contribute to allergenic cross-reactivity among specific pollens and foods

Item Type:	Journal Article
Publication:	Journal Of Allergy And Clinical Immunology
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	Parthenium;glycoprotein;allergen;hydroxyproline.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	12 Aug 2009 11:03
Last Modified:	19 Sep 2010 05:26
URI:	http://eprints.iisc.ac.in/id/eprint/19005

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