Dual regulation of heat-stable enterotoxin-mediated cGMP accumulation in T84 cells by receptor desensitization and increased phosphodiesterase activity

Bakre, MM and Visweswariah, SS (1997) Dual regulation of heat-stable enterotoxin-mediated cGMP accumulation in T84 cells by receptor desensitization and increased phosphodiesterase activity. In: Febs Letters, 408 (3). pp. 345-349. (In Press)

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Abstract

Abstract: We report the regulation of cGMP accumulation induced by the heat-stable enterotoxin, STh, in the T84 human colonic cell line, STh binding to its receptor, guanylyl cyclase C (GCC), leads to elevated intracellular levels of cGMP, Prolonged exposure of T84 cells to STh induced refractoriness to further cGMP accumulation, without significant receptor internalization, but with reduced STh-induced cGMP synthesis by the receptor. Significantly, increased degradation of cGMP by a cGMP-specific phosphodiesterase was observed in desensitized cells, This is the first report on the desensitization of GCC, as well as the role of the Type V phosphodiesterase in inducing cellular refractoriness. (C) 1997 Federation of European Biochemical Societies.

Item Type:	Journal Article
Publication:	Febs Letters
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Federation of European Biochemical Societies.
Keywords:	Guanylyl cyclase C;heat-stable enterotoxins;cellular refractoriness;cGMP-binding, cGMP-specific;phosphodiesterase 31. VISWESWARIAH SS INTERACTION OF HEAT-STABLE ENTEROTOXINS WITH HUMAN COLONIC-(T84) CELLS - MODULATION OF THE ACTIVATION OF GUANYLYL CYCLASE MICROBIAL PATHOGENESIS 12 : 209 1992
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	03 Jul 2009 10:34
Last Modified:	19 Sep 2010 05:25
URI:	http://eprints.iisc.ac.in/id/eprint/18873

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