Methyl angolensate, a natural tetranortriterpenoid induces intrinsic apoptotic pathway in leukemic cells

Chiruvella, Kishore K and Kari, Vijayalakshmi and Choudhary, Bibha and Nambiar, Mridula and Ghanta, Rama Gopal and Raghavan, Sathees C (2008) Methyl angolensate, a natural tetranortriterpenoid induces intrinsic apoptotic pathway in leukemic cells. In: FEBS Letters, 582 (29). pp. 4066-4076.

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Abstract

Methyl angolensate (MA), a natural tetranortriterpenoid, purified from Soymida febrifuga is examined for the first time for its anticancer properties. We find that MA inhibits growth of T-cell leukemia and chronic myelogenous leukemia cells in a time- and dose-dependent manner. Accumulation of cells in the subG1 peak, annexin V binding and DNA fragmentation suggested induction of apoptosis. Besides, upregulation of BAD (proapoptotic) and downregulation of BCL2 (antiapoptotic) gene products further supported induction of apoptosis. Loss of mitochondrial membrane potential, activation of caspase 9, caspase 3, cleavage of PARP, downregulation of Ku70/80 and phosphorylation of MAP kinases suggested that MA could induce intrinsic pathway of apoptosis in leukemic cells.

Item Type:	Journal Article
Publication:	FEBS Letters
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	Cytotoxicity;Double-strand break;Cancer therapeutics; Chemotherapy;Callus; DNA damage.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	21 Aug 2009 11:06
Last Modified:	19 Sep 2010 04:57
URI:	http://eprints.iisc.ac.in/id/eprint/17495

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