In Vivo and in Vitro Destruction of Rat Liver Cytochrome P-450 by a Monoterpene Ketone, Pulegone

Madyastha, Prema and Moorthy, B and Vaidyanathanf, CS and Madyastha, KM (1985) In Vivo and in Vitro Destruction of Rat Liver Cytochrome P-450 by a Monoterpene Ketone, Pulegone. In: Biochemical and Biophysical Research Communications, 128 (2). pp. 921-927.

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Abstract

In vivo administration of pulegone once daily decreased the levels of liver microsomal cvt. P-450 to the extent of 32 and 76% at the end of 24 and 96 hrs respectively. However, cyt. $b_5$ and NAD(P)H-cyt. c reductase activities remained unchanged. In vitro incubation T15 min) of liver microsomes from phenobarbital mtreated rats with pulegone (10 mM), aerobically or anaerobically resulted in the loss (\sim 60%) of cyt. P-450 in the presence or absence of NADPH. Destruction of cyt. P-450 was more in PBtreated microsomes as compared to 3-methylcholanthrene (MC)-treated and control microsomes, The loss of cyt. P-450 was accompanied by a concomitant loss of microsomal heme. In contrast, menthone or carvone upon incubation with PB-induced microsomes resulted in the conversion (25-40%) of cyt. P-450 to cyt. P-420 without any loss of microsomal heme, The destructive process is irreversible, time dependent, linear upto a substrate concentration of 10 mNLa nd follows first order kinetics.

Item Type:	Journal Article
Publication:	Biochemical and Biophysical Research Communications
Publisher:	Elsevier
Additional Information:	Copyright of this article belongs to Elsevier.
Department/Centre:	Division of Chemical Sciences > Organic Chemistry Division of Biological Sciences > Biochemistry
Date Deposited:	16 Jul 2008
Last Modified:	19 Sep 2010 04:47
URI:	http://eprints.iisc.ac.in/id/eprint/14973

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